| Expression pattern: |
UP |
| Associated gene: |
ABCB1, DYNC1H1 |
| Associated microRNA: |
miR-198 |
| Biological function: |
circ_0002060 promotes doxorubicin resistance and OS progression; circ_0002060 silencing reduces DOX resistance, suppresses proliferation, and promotes apoptosis in DOX-resistant OS cells. |
| Molecular mechanism: |
circ_0002060 functions as a miR-198 sponge to upregulate ABCB1 expression, thereby enhancing DOX resistance in osteosarcoma. |
| Biological pathway or process: |
drug resistance (promotes); chemoresistance (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0002060 expression is associated with poor response to DOX treatment and poor overall survival in OS patients; circ_0002060 may be a promising biomarker for OS therapy. |
| Description: |
circ_0002060 is upregulated in osteosarcoma tissues and DOX-resistant osteosarcoma cells, and high expression is associated with poor prognosis and poor treatment response. Mechanistically, circ_0002060 sponges miR-198 to increase ABCB1 expression, thereby promoting doxorubicin resistance, proliferation, and OS progression while inhibiting apoptosis. |
| Confidence score: |
0.8436 |