| Expression pattern: |
UP |
| Associated gene: |
KAT5, c-MYC, histone H3 lysine 27 acetylation (H3K27ac), RNA polymerase II |
| Associated microRNA: |
- |
| Biological function: |
promotes NSCLC cell proliferation and viability; inhibits apoptosis; promotes cell cycle progression; promotes tumor growth in vivo |
| Molecular mechanism: |
circRHOT1 directly interacts with KAT5 and recruits it to the c-MYC promoter to enhance H3K27ac and RNA polymerase II enrichment, thereby epigenetically increasing c-MYC transcription/expression |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; FISH / smFISH; Transfection; MTT; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; ChIP / ChIP-seq; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); TUNEL |
| Clinical significance: |
- |
| Description: |
circRHOT1 is up-regulated in NSCLC tumor tissues and lung cancer cell lines and promotes proliferation and tumor growth while suppressing apoptosis. Mechanistically, circRHOT1 binds KAT5 and facilitates its recruitment to the c-MYC promoter, increasing transcription-associated H3K27ac/RNA polymerase II enrichment and elevating c-MYC expression. |
| Confidence score: |
0.8151 |