circRNA basic information
circBase ID: -
Name: rno_circ_PVT1
Synonym: CircPVT1
Host Gene: PVT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005380
MONDO name: osteonecrosis
Disease details: Steroid-induced osteonecrosis of the femoral head
Disease DO ID:
 0080008, 10159
Disease MeSH ID:
 D010020
Disease NCIt ID:
 C34880; C35476
Disease ICD11 ID:
 536467755
Disease OMIM ID:
 -
Species: Rat
Species details: Rattus norvegicus
Tissue specimen:

femoral head
Cell lines:

-
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: Smad7, TGFbeta, Smad2/3
Associated microRNA: miR-21-5p
Biological function: Promotes osteogenic differentiation and proliferation and inhibits apoptosis of GC-treated BMSCs; attenuates osteonecrosis in SIONFH rats.
Molecular mechanism: Acts as a ceRNA sponging miR-21-5p to upregulate Smad7 and inhibit GC-activated TGFbeta/Smad2/3 signaling.
Biological pathway or process:

TGF-beta/SMAD (inhibits); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Luciferase Reporter Assay; RNA Pull-Down; Transfection; BrdU; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

-
Description:

CircPVT1 is down-regulated in SIONFH models. Its overexpression protects against steroid/GC-induced osteonecrosis phenotypes by promoting osteogenic differentiation and proliferation while inhibiting apoptosis. Mechanistically, CircPVT1 sponges miR-21-5p to increase Smad7 and suppress TGFbeta/Smad2/3 signaling.
Confidence score:

0.7045
Other information
Title:

CircPVT1 up-regulation attenuates steroid-induced osteonecrosis of the femoral head through regulating miR-21-5p-mediated Smad7/TGFbeta signalling pathway.

Journal: Journal of cellular and molecular medicine
Published: 2021
PubMed ID: 33733589
Study type:

combined biological and clinical study
Data availability: The data sets used and analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -