circRNA basic information
circBase ID: hsa_circ_0001013
Name: -
Synonym: hsa-circ-000684
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent normal tissues; normal mucosal tissues; tumor tissues
Cell lines:

NCI-N87; MGC-803; SGC-7901; BGC-823; BEL-7402; GES-1; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ZEB1, Ago2, VEGFA, CD31
Associated microRNA: miR-186
Biological function: Promotes GC cell proliferation, migration, invasion, angiogenesis/tube formation, xenograft tumor growth, and GC progression; knockdown inhibits these malignant phenotypes.
Molecular mechanism: hsa-circ-000684 acts as a ceRNA/miR-186 sponge, reducing miR-186 activity/expression and increasing ZEB1 expression, thereby promoting GC progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); metastasis (promotes)
Detected method:
Q
B
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; Northern Blot; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Transwell Assay; Wound Healing Assay; Tube Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

hsa-circ-000684 expression was significantly correlated with lymph node metastasis and TNM staging in GC patients; proposed as a potential therapeutic target.
Description:

hsa-circ-000684 is upregulated in gastric cancer tissues and cell lines. It promotes GC proliferation, migration, invasion, angiogenesis/tube formation, and xenograft growth by acting as a miR-186 sponge and increasing ZEB1 expression. Its expression correlates with lymph node metastasis and TNM stage, suggesting biological and potential therapeutic relevance.
Confidence score:

0.866
Other information
Title:

Oncogenic circular RNA Hsa-circ-000684 interacts with microRNA-186 to upregulate ZEB1 in gastric cancer.

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Published: 2020
PubMed ID: 32388910
Study type:

combined biological and clinical study
Data availability: GSE83521; GSE49051; circBase; CircInteractome; mirDIP; starBase; MicroT; STRING
Code availability: -