| Expression pattern: |
UP |
| Associated gene: |
ITGB8 |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
Promotes NSCLC cell proliferation, colony formation, migration, invasion, metastasis and tumor growth in vivo. |
| Molecular mechanism: |
circ_0017956 acts as a miR-515-5p sponge to upregulate ITGB8 expression through a ceRNA mechanism. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model |
| Clinical significance: |
Higher circ_0017956 expression was associated with lymph node metastasis and TNM stage III in NSCLC patients. |
| Description: |
circ_0017956 is up-regulated in NSCLC tissues and cell lines and is associated with lymph node metastasis and advanced TNM stage. Functionally, circ_0017956 promotes NSCLC proliferation, migration, invasion, metastasis and xenograft tumor growth. Mechanistically, it mainly localizes to the cytoplasm and acts as a ceRNA that sponges miR-515-5p to increase ITGB8 expression. |
| Confidence score: |
0.7519 |