circRNA basic information
circBase ID: hsa_circ_0124554
Name: hsa_circ_FOXP1
Synonym: circ_0124554
Host Gene: FOXP1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent tumor tissues; normal tissues; plasma
Cell lines:

DLD-1; HCT 116; HT 29; LoVo; Caco 2; Caco-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AKT, GSK-3beta, mTOR
Associated microRNA: -
Biological function: promotes invasion, migration and metastasis (including liver metastasis) in colorectal cancer, and is associated with vascular invasion
Molecular mechanism: directly binds AKT protein, blocks AKT ubiquitination, increases AKT phosphorylation and activates downstream signaling
Biological pathway or process:

PI3K/AKT (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ubiquitination (inhibits)
Detected method:
Q
B
H
M
Validation methods:

Microarray; RNase R Treatment; RT-qPCR; Sanger Sequencing; FISH / smFISH; Northern Blot; ISH (In Situ Hybridization); Bioinformatics Analysis; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Wound Healing Assay; CCK8; Cell Cycle Assay; In Vivo Animal Model; H&E Staining; Western Blot; Survival Analysis; Clinical Sample Validation
Clinical significance:

Higher expression correlates with vascular invasion and liver metastasis, predicts poorer prognosis, and plasma circ-LNLM could distinguish LNLM1 from LNLM0
Description:

In lymph node-negative CRC with synchronous liver metastasis (LNLM1), hsa_circ_0124554 is up-regulated and promotes invasion/migration and in vivo metastasis. Mechanistically, it directly binds AKT, inhibits AKT ubiquitination, sustains AKT phosphorylation, and activates AKT-associated downstream signaling (e.g., GSK-3beta and mTOR). Clinically, higher expression is associated with vascular invasion/liver metastasis and poorer prognosis, and plasma levels may help distinguish LNLM1 from LNLM0.
Confidence score:

0.8551
Other information
Title:

CircRNA circ_0124554 blocked the ubiquitination of AKT promoting the skip lymphovascular invasion on hepatic metastasis in colorectal cancer.

Journal: Cell death & disease
Published: 2021
PubMed ID: 33723214
Study type:

combined biological and clinical study
Data availability: E-MTAB-8831
Code availability: -