| Expression pattern: |
UP |
| Associated gene: |
IRS2 |
| Associated microRNA: |
miR-613 |
| Biological function: |
circFAM126A promotes NSCLC cell proliferation, migration, invasion, cell cycle progression and tumor growth, and inhibits apoptosis; knockdown of circFAM126A suppresses these malignant phenotypes and induces apoptosis and cell cycle arrest. |
| Molecular mechanism: |
circFAM126A functions as a miRNA sponge for miR-613 to upregulate IRS2 expression, thereby promoting NSCLC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circFAM126A expression is associated with worse overall survival in NSCLC patients and may be helpful for NSCLC diagnosis. |
| Description: |
circFAM126A is up-regulated in NSCLC tissues and cells and high expression is associated with poorer survival. Functionally, circFAM126A acts as an oncogenic circRNA that promotes proliferation, migration, invasion, cell cycle progression, EMT-related marker changes and xenograft tumor growth while suppressing apoptosis. Mechanistically, it sponges miR-613 to upregulate IRS2, forming a circFAM126A/miR-613/IRS2 regulatory axis. |
| Confidence score: |
0.8712 |