| Expression pattern: |
UP |
| Associated gene: |
Notch1, Ago2, P21, c-Met, SNAIL, VEGF |
| Associated microRNA: |
miR-34a |
| Biological function: |
promotes tumor invasion, proliferation and angiogenesis; promotes tumorigenesis and liver metastasis in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-34a to increase Notch1 expression and activate Notch 1 pathway; AGO2-associated RISC binding supports the sponge mechanism |
| Biological pathway or process: |
Notch (promotes); proliferation (promotes); invasion (promotes); angiogenesis (promotes); migration (promotes); cell cycle (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Transwell Assay; EdU Staining; Cell Cycle Assay; CCK8; Tube Formation Assay; Western Blot; ELISA; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; Microarray |
| Clinical significance: |
High circ-ASH2L expression was correlated with lymphatic invasion and TNM stage and was an independent risk factor for PDAC patient overall survival; may be a useful biomarker of PDAC diagnosis or progression |
| Description: |
circ-ASH2L is up-regulated in PDAC tissues/cell lines and mainly localizes to the cytoplasm. It promotes invasion, proliferation, cell-cycle progression and angiogenesis by sponging miR-34a to relieve repression of Notch1 (Notch signaling activation), and high expression predicts worse overall survival. |
| Confidence score: |
0.8871 |