| Expression pattern: |
UP |
| Associated gene: |
PI3K/AKT pathway, MEK/ERK pathway |
| Associated microRNA: |
miR-760 |
| Biological function: |
promotes cell proliferation and invasion; inhibits apoptosis; promotes tumor growth and EMT in vivo |
| Molecular mechanism: |
direct binding/sponging of miR-760, modulating PI3K/AKT and MEK/ERK signaling |
| Biological pathway or process: |
PI3K/AKT (promotes); ERK (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Transfection; CCK8; TUNEL; Transwell Assay; Colony Formation Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
related to NSCLC development and lymph node metastasis (LNM) |
| Description: |
circ_0008594 is up-regulated in NSCLC and is associated with lymph node metastasis. It promotes proliferation and invasion and suppresses apoptosis, at least partly by directly binding (sponging) miR-760 and activating PI3K/AKT and MEK/ERK signaling; these effects were supported by in vitro assays and an H23 cell line-derived xenograft model. |
| Confidence score: |
0.7373 |