| Expression pattern: |
UP |
| Associated gene: |
PDPK1 |
| Associated microRNA: |
miR-1183 |
| Biological function: |
Promotes NSCLC cell proliferation and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circRNA-CPA4 acts as a molecular sponge for miR-1183 to up-regulate PDPK1 expression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
- |
| Description: |
circRNA-CPA4 is up-regulated in NSCLC cell lines and functions as an oncogenic circRNA. It promotes proliferation and tumor growth while suppressing apoptosis by sponging miR-1183 and increasing PDPK1 expression. Knockdown of circRNA-CPA4 inhibits NSCLC cell proliferation and xenograft growth and promotes apoptosis. |
| Confidence score: |
0.7295 |