| Expression pattern: |
UP |
| Associated gene: |
FLI1, c-Myc, IGFBP3 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
promotes osteosarcoma cell migration, invasion, and proliferation; inhibits apoptosis; promotes tumorigenesis in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) by binding miR-145-5p in the cytoplasm, relieving repression of FLI1 and affecting downstream genes (c-Myc, IGFBP3). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
positively correlated with osteosarcoma development; could be valuable markers to predict the prognosis of patients with osteosarcoma |
| Description: |
circCAMSAP1 (hsa_circ_0004338) is up-regulated in osteosarcoma tissues/cell lines and mainly localized in the cytoplasm. It promotes osteosarcoma proliferation, migration, invasion and tumor growth by sponging miR-145-5p to derepress FLI1 (with downstream effects including increased c-Myc and decreased IGFBP3). |
| Confidence score: |
0.8233 |