| Expression pattern: |
UP |
| Associated gene: |
NOTCH2 |
| Associated microRNA: |
miR-335 |
| Biological function: |
circHECTD1 knockdown inhibited OGD/R-induced EndoMT in HCMECs and promoted OGD/R-induced migration and tube formation; circHECTD1 is implicated as a modulator of ischemic stroke progression. |
| Molecular mechanism: |
circHECTD1 acts through a ceRNA-like mechanism by sponging miR-335 to indirectly regulate NOTCH2 expression, thereby modulating EndoMT in ischemic stroke models. |
| Biological pathway or process: |
ceRNA regulation (promotes); EMT (promotes); migration (inhibits); angiogenesis (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; Transwell Assay; Tube Formation Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circHECTD1 might act as a potential target against IS. |
| Description: |
This study found that circHECTD1 is upregulated in ischemic stroke models, including MCAO mouse brain tissues and OGD/R-treated HCMECs. Functional experiments indicate that circHECTD1 knockdown suppresses OGD/R-induced EndoMT and affects endothelial migration and tube formation. Mechanistically, circHECTD1 sponges miR-335 to regulate NOTCH2, suggesting a circHECTD1/miR-335/NOTCH2 axis in ischemic stroke-related endothelial dysfunction. |
| Confidence score: |
0.627 |