| Expression pattern: |
UP |
| Associated gene: |
ROBO1, c-Myc, Bcl-2, GLUT1, LDHA |
| Associated microRNA: |
miR-130a-5p |
| Biological function: |
circROBO1 promotes liver cancer cell viability, proliferation, antiapoptosis ability, Warburg effect, and malignant progression; circROBO1 antagonizes matrine-mediated inhibition of liver cancer progression. |
| Molecular mechanism: |
circROBO1 acts as a cytoplasmic miRNA sponge for miR-130a-5p to upregulate ROBO1 expression; matrine suppresses liver cancer progression by regulating the circROBO1/miR-130a-5p/ROBO1 axis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes); drug resistance (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circROBO1 was upregulated in human clinical liver cancer tumors and may be a molecular target in matrine-based liver cancer therapy. |
| Description: |
circROBO1 is upregulated in liver cancer tissues and functions as an oncogenic circRNA. It promotes liver cancer cell viability, proliferation, antiapoptosis capacity, and Warburg effect by sponging miR-130a-5p to increase ROBO1 expression. Matrine suppresses liver cancer progression partly by downregulating circROBO1 and inhibiting the circROBO1/miR-130a-5p/ROBO1 axis. |
| Confidence score: |
0.7855 |