| Expression pattern: |
DN |
| Associated gene: |
TRAF6 |
| Associated microRNA: |
miR-182 |
| Biological function: |
inhibits migration and invasion; promotes cell cycle arrest; promotes apoptosis; inhibits tumor growth; exosomal circSPATA6 inhibits OSCC cell progression |
| Molecular mechanism: |
ceRNA sponge mechanism: circSPATA6 sponges miR-182 to relieve repression of TRAF6; validated by dual-luciferase reporter and Ago2-RIP |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); cell cycle (inhibits); apoptosis (promotes); proliferation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; ROC Analysis; Clinical Sample Validation |
| Clinical significance: |
circSPATA6 is downregulated in OSCC serum and shows diagnostic value with AUC=0.7748 in ROC analysis |
| Description: |
circSPATA6 is down-regulated in OSCC serum and cell lines and acts as a tumor suppressor. It sponges miR-182 to upregulate TRAF6, thereby inhibiting migration/invasion, inducing cell-cycle arrest and apoptosis, and suppressing xenograft tumor growth; exosomal circSPATA6 also restrains OSCC cell progression. |
| Confidence score: |
0.8005 |