| Expression pattern: |
UP |
| Associated gene: |
FXR1, Fbxo4 |
| Associated microRNA: |
miR-503-3p, miR-503-5p |
| Biological function: |
promotes keloid-derived fibroblast proliferation and extracellular matrix accumulation; inhibits apoptosis; facilitates keloid progression |
| Molecular mechanism: |
circNRIP1 binds FXR1 and stabilizes it by blocking Fbxo4-mediated FXR1 ubiquitination and proteasomal degradation, thereby promoting pre-miR-503 maturation and increasing miR-503-3p/miR-503-5p |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Co-IP; Northern Blot |
| Clinical significance: |
circNRIP1 has potential as a novel therapeutic target for the control and/or treatment of keloids. |
| Description: |
circNRIP1 is up-regulated in keloid tissues and keloid-derived fibroblasts and promotes fibroblast proliferation and extracellular matrix accumulation while inhibiting apoptosis. Mechanistically, circNRIP1 binds FXR1 and blocks Fbxo4-mediated ubiquitination/degradation of FXR1, which enhances pre-miR-503 maturation and increases miR-503-3p/miR-503-5p levels, facilitating keloid progression. |
| Confidence score: |
0.7977 |