| Expression pattern: |
UP |
| Associated gene: |
MAPK1, AGO2 |
| Associated microRNA: |
miR-6805-3p |
| Biological function: |
Promotes cervical cancer angiogenesis, HUVEC migration and invasion, tumor growth, and metastasis. |
| Molecular mechanism: |
Tumor cell-derived exosomal circ_0064516 acts as a ceRNA sponge for miR-6805-3p, thereby upregulating MAPK1 expression and promoting angiogenesis and tumor growth. |
| Biological pathway or process: |
MAPK (promotes); angiogenesis (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; Tube Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
The increased expression of circ_0064516 in cervical cancer may serve as a biomarker for early diagnosis and prognostic evaluation. |
| Description: |
This study identifies circ_0064516/hsa_circ_0064516, derived from ANKRD28, as an up-regulated exosomal circRNA in cervical cancer. Tumor cell-derived exosomes transfer circ_0064516 into HUVECs, where it sponges miR-6805-3p to elevate MAPK1, thereby promoting endothelial migration, angiogenesis, tumor growth, and metastasis in cervical cancer models. |
| Confidence score: |
0.8278 |