| Expression pattern: |
UP |
| Associated gene: |
ATG7, ATG14, DSC1, ATG101, FTO, ATG5 |
| Associated microRNA: |
miR-129 / miR-129-5p |
| Biological function: |
Promotes autophagic flux, proliferation, invasion/migration, and metastasis in epithelial ovarian cancer cells. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-129 to regulate ATG7/ATG14; directly binds DSC1 protein to facilitate DSC1-ATG101 interaction; binds FTO mRNA and promotes FTO expression, affecting m6A methylation of ATG5/ATG7 mRNA. |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); m6A modification (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; EdU Staining; Transwell Assay; Western Blot; Co-IP; In Vivo Animal Model |
| Clinical significance: |
May serve as a candidate biomarker/marker for EOC diagnosis and treatment. |
| Description: |
circRAB11FIP1 is upregulated in epithelial ovarian cancer and promotes autophagic flux, enhancing proliferation, invasion/migration and in vivo metastasis. Mechanistically, it sponges miR-129 to increase ATG7/ATG14, binds DSC1 to enhance DSC1-ATG101 interaction, and binds FTO mRNA to elevate FTO and modulate m6A-dependent ATG5/ATG7 expression; it is proposed as a diagnostic/therapeutic biomarker for EOC. |
| Confidence score: |
0.7836 |