| Expression pattern: |
UP |
| Associated gene: |
IGF1R, Ago2 |
| Associated microRNA: |
miR-378a-3p |
| Biological function: |
promotes proliferation; promotes invasion; promotes colony formation; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA sponge of miR-378a-3p to upregulate IGF1R and activate IGF1R/PI3K/AKT signaling |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Transwell Assay; Colony Formation Assay; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis; Western Blot |
| Clinical significance: |
Increased expression of circPDHX was associated with Gleason score and pathogenic T stage and acted as an independent prognostic factor of poor survival in patients with PCa. |
| Description: |
circPDHX (hsa_circ_0003768), derived from PDHX, is up-regulated in prostate cancer and mainly localized in the cytoplasm. It promotes PCa cell proliferation and invasion by sponging miR-378a-3p, thereby increasing IGF1R expression and activating IGF1R/PI3K/AKT signaling, and its high expression predicts poor overall survival. |
| Confidence score: |
0.8655 |