| Expression pattern: |
UP |
| Associated gene: |
PHF21B, beta-catenin, PD-L1 |
| Associated microRNA: |
hsa-miR-527 |
| Biological function: |
promotes proliferation, migration, invasion, stemness-like properties, metastasis and chemoresistance; contributes to anti-PD-L1 therapy resistance |
| Molecular mechanism: |
ceRNA sponge of miR-527 to relieve repression of PHF21B, promoting downstream beta-catenin/Wnt signaling and stemness; influences PD-L1 levels |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); chemoresistance (promotes); apoptosis (inhibits); Wnt/beta-catenin (promotes); ceRNA regulation (promotes); drug resistance (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; ISH (In Situ Hybridization); Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); TUNEL; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Higher levels of hsa_circ_0003222 were associated with the stage, metastasis, and survival rate of patients with NSCLC; high expression indicates unfavorable prognosis. |
| Description: |
hsa_circ_0003222 is up-regulated in NSCLC tissues and lung cancer stem-like cells and promotes proliferation, migration/invasion, stemness and cisplatin resistance. Mechanistically, it sponges miR-527 to increase PHF21B and downstream beta-catenin/Wnt signaling, and its inhibition sensitizes tumors to anti-PD-L1 therapy in vivo. |
| Confidence score: |
0.8512 |