circRNA basic information
circBase ID: hsa_circ_0025508
Name: hsa_circ_GPRC5A
Synonym: -
Host Gene: Gprc5a
Genomic location(hg19): chr12:13065017-13066600:+
Genomic location(hg38): chr12:12912083-12913666:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; paired normal tissues

Cell lines:

MHCC97; PLC; SK-Hep1; Huh7; Hep3B; HepG2; LO2; 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: YAP1, TEAD1, Ago2
Associated microRNA: miR-1283
Biological function: promotes HCC progression and cell proliferation; inhibits apoptosis; promotes tumor growth in vivo
Molecular mechanism: Acts as a miR-1283 sponge to increase YAP1/TEAD1 expression and activate YAP1/TEAD1 (Hippo-related) signaling
Biological pathway or process:

Hippo/YAP (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

divergent primers PCR; RT-qPCR; Clinical Sample Validation; Survival Analysis; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; CCK8; BrdU; Colony Formation Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

High circGprc5a expression is associated with reduced overall survival and correlates with tumor size and differentiation stage in HCC patients

Description:

circGprc5a is up-regulated in HCC tissues/cell lines and its high level is associated with poorer overall survival. Functionally, circGprc5a promotes HCC cell proliferation and tumor growth while inhibiting apoptosis by sponging miR-1283 and thereby increasing YAP1/TEAD1 expression/activity.

Confidence score:

0.8067

Other information
Title:

Circular RNA Gprc5a Promotes HCC Progression by Activating YAP1/TEAD1 Signalling Pathway by Sponging miR-1283.

Journal: OncoTargets and therapy
Published: 2020
PubMed ID: 32547082
Study type:

combined biological and clinical study

Data availability: -
Code availability: -