| Expression pattern: |
UP |
| Associated gene: |
MACC1, Ago2 |
| Associated microRNA: |
miR-138-5p |
| Biological function: |
promotes proliferation, migration and invasion; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA/miRNA sponge: circ_0006174 sponges miR-138-5p to upregulate MACC1 expression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNA-seq; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high expression of circ_0006174 had a shorter overall survival |
| Description: |
circ_0006174 is up-regulated in colorectal cancer tissues/cells and acts as an oncogenic circRNA. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-138-5p, thereby increasing MACC1 expression to promote proliferation, migration/invasion and tumor growth while inhibiting apoptosis; high circ_0006174 correlates with poorer overall survival. |
| Confidence score: |
0.8035 |