| Expression pattern: |
UP |
| Associated gene: |
ARL2 |
| Associated microRNA: |
miR-646 |
| Biological function: |
promotes cell growth, cell cycle progression and secretion of inflammatory mediators in osteosarcoma cells |
| Molecular mechanism: |
ceRNA mechanism: circ_0000527 sponges miR-646 to increase ARL2 expression |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Cell Cycle Assay; ELISA |
| Clinical significance: |
- |
| Description: |
circ_0000527 is up-regulated in osteosarcoma cells and patient specimens. Functionally, its overexpression promotes proliferation and cell-cycle progression and increases secretion of inflammatory cytokines. Mechanistically, circ_0000527 acts as a miR-646 sponge to relieve repression of ARL2 (circ_0000527/miR-646/ARL2 axis). |
| Confidence score: |
0.6996 |