circRNA basic information
circBase ID: hsa_circ_0068515
Name: hsa_circ_TP63
Synonym: CircTP63
Host Gene: TP63
Genomic location(hg19): chr3:189590647-189604340:+
Genomic location(hg38): chr3:189872858-189886551:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005411
MONDO name: gallbladder cancer
Disease details: gallbladder cancer
Disease DO ID:
 3121
Disease MeSH ID:
 -
Disease NCIt ID:
 C7481
Disease ICD11 ID:
 922082116
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

GBC tissues; adjacent normal tissues; xenograft tumors; lung metastatic nodules
Cell lines:

NOZ; GBC-SD; SGC-996; HIBEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EZH2
Associated microRNA: miR-217
Biological function: promotes cell proliferation, cell cycle progression, migration, invasion, EMT, tumor growth and metastasis in gallbladder cancer
Molecular mechanism: ceRNA mechanism: circTP63 sponges miR-217 to de-repress EZH2 expression, facilitating tumor progression
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

Higher circTP63 expression was associated with lymph node metastasis and short overall survival (OS) in patients with GBC; circTP63 expression could be a prognostic maker for GBC.
Description:

circTP63 is up-regulated in gallbladder cancer tissues and cell lines and promotes proliferation, cell-cycle progression, migration/invasion and EMT. It acts as a ceRNA by sponging miR-217 to increase EZH2 expression, thereby facilitating tumor progression; high circTP63 predicts poorer overall survival and is associated with lymph node metastasis.
Confidence score:

0.8227
Other information
Title:

CircTP63 promotes cell proliferation and invasion by regulating EZH2 via sponging miR-217 in gallbladder cancer.

Journal: Cancer cell international
Published: 2021
PubMed ID: 34789260
Study type:

combined biological and clinical study
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -