| Expression pattern: |
UP |
| Associated gene: |
GUCD1, Ago2, HK2 |
| Associated microRNA: |
miR-29a-3p |
| Biological function: |
promotes HCC cell proliferation, migration, invasion and glycolysis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA sponge mechanism: circ-ZNF652 sponges miR-29a-3p to regulate GUCD1 expression, affecting glycolysis (HK2) and malignant phenotypes |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
Potential serum exosomal biomarker for HCC (elevated in patient serums) |
| Description: |
Exosomal circ-ZNF652 is up-regulated in HCC patient serum exosomes and HCC cells. It mainly localizes in the cytoplasm and promotes proliferation, migration, invasion, glycolysis and in vivo tumor growth by sponging miR-29a-3p to upregulate GUCD1 (with downstream effects including HK2-associated glycolysis). |
| Confidence score: |
0.762 |