circRNA basic information
circBase ID: hsa_circ_0001727
Name: hsa_circ_ZKSCAN1
Synonym: -
Host Gene: ZKSCAN1
Genomic location(hg19): chr7:99621041-99621930:+
Genomic location(hg38): chr7:100023418-100024307:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

hepatocellular carcinoma tissue; adjacent nontumorous liver tissue
Cell lines:

Huh7; SMMC-7721; BEL-7402; HepG2; Hep3B; L02; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: RAC2, EFNA3, caspase 3, TGFB1, ITGB4, CXCR4, survivin, CCND1, COL3A1, CDH5, MYB, PDK1, BCL2
Associated microRNA: -
Biological function: inhibits hepatocellular carcinoma cell proliferation, migration, invasion and suppresses tumor growth in vivo
Molecular mechanism: may function as a ceRNA and regulate proliferation- and invasion-related signaling pathways; knockdown enriched differentially expressed genes in PI3K and migration-related pathways
Biological pathway or process:

PI3K/AKT (other); migration (inhibits); invasion (inhibits); proliferation (inhibits); apoptosis (other); ceRNA regulation (other)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; RNA-seq; FISH / smFISH; Clinical Sample Validation; Transfection; MTT; Transwell Assay; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis
Clinical significance:

circZKSCAN1 expression could serve as a biomarker for distinguishing cancerous tissue from adjacent noncancerous liver tissue; cirZKSCAN1 might be a useful marker for the diagnosis of HCC
Description:

circZKSCAN1 is down-regulated in hepatocellular carcinoma tissues and cell lines and acts as a tumor suppressor by inhibiting proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, it is mainly cytoplasmic and is proposed to act as a ceRNA affecting PI3K- and migration-related gene programs, and it shows diagnostic potential (AUC 0.834) for distinguishing tumor from adjacent tissue.
Confidence score:

0.7999
Other information
Title:

ZKSCAN1 gene and its related circular RNA (circZKSCAN1) both inhibit hepatocellular carcinoma cell growth, migration, and invasion but through different signaling pathways.

Journal: Molecular oncology
Published: 2017
PubMed ID: 28211215
Study type:

combined biological and clinical study
Data availability: -
Code availability: -