| Expression pattern: |
DN |
| Associated gene: |
LIFR |
| Associated microRNA: |
miR-543 |
| Biological function: |
Suppresses HCC cell proliferation, invasion, and migration (tumor suppressor). |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-543 to indirectly upregulate LIFR (ceRNA mechanism). |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; Luciferase Reporter Assay; RNA Pull-Down; FISH / smFISH; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis; Western Blot |
| Clinical significance: |
Reduced expression of circARPP21 in HCC patients is correlated with larger tumor size, higher tug-lymph node metastasis (TNM) stage, and poor prognosis as indicated by elevated levels of alpha-fetoprotein (AFP). |
| Description: |
circARPP21 (hsa_circ_0123629) is downregulated in hepatocellular carcinoma and functions as a tumor suppressor by sponging miR-543, thereby relieving miR-543-mediated repression of LIFR. Increasing circARPP21 inhibits HCC cell proliferation, migration, and invasion, and low circARPP21 expression is associated with worse clinical features and prognosis. |
| Confidence score: |
0.8658 |