circRNA basic information
circBase ID: hsa_circ_0001427
Name: hsa_circ_PDLIM5
Synonym: circRNA17
Host Gene: PDLIM5
Genomic location(hg19): chr4:95561426-95578698:+
Genomic location(hg38): chr4:94640275-94657547:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

prostate tissue
Cell lines:

C4-2; CWR22Rv1; 293T; HEK-293
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: ARv7, androgen receptor / AR
Associated microRNA: hsa-miR-181c-5p, hsa-miR-186-5p, hsa-miR-320a, hsa-miR-1, hsa-miR-320b, hsa-miR-138-5p, hsa-miR-320c, hsa-miR-181a-5p, hsa-miR-320d, hsa-miR-181b-5p, hsa-miR-370-3p, hsa-miR-181d-5p, hsa-miR-4262, hsa-miR-4429, hsa-miR-206, hsa-miR-494-3p, hsa-miR-27a-3p, hsa-miR-613, hsa-miR-27b-3p
Biological function: Suppresses enzalutamide resistance, cell invasion, tumor growth and metastasis by repressing ARv7 via stabilizing miR-181c-5p.
Molecular mechanism: Enzalutamide suppresses transcription of host gene PDLIM5 to reduce circRNA17; circRNA17 physically interacts with and stabilizes miR-181c-5p (reservoir/stabilizing factor), increasing miR-181c-5p activity; miR-181c-5p directly binds ARv7 3′UTR to repress ARv7, thereby reducing Enz resistance and invasion.
Biological pathway or process:

invasion (inhibits); metastasis (inhibits); proliferation (inhibits); ceRNA regulation (other); drug resistance (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis; ChIP / ChIP-seq; Western Blot; Clinical Sample Validation
Clinical significance:

Lower circRNA17 expression is associated with higher Gleason score prostate cancer.
Description:

circRNA17 is down-regulated in enzalutamide-resistant CRPC and high-Gleason-score prostate cancer. It suppresses ARv7-driven enzalutamide resistance and invasion by interacting with and stabilizing miR-181c-5p, which directly targets the ARv7 3′UTR; enzalutamide represses circRNA17 via transcriptional suppression of its host gene PDLIM5.
Confidence score:

0.8166
Other information
Title:

Preclinical study using circular RNA 17 and micro RNA 181c-5p to suppress the enzalutamide-resistant prostate cancer progression.

Journal: Cell death & disease
Published: 2019
PubMed ID: 30674872
Study type:

combined biological and clinical study
Data availability: Supplementary Information accompanies this paper at (10.1038/s41419-018-1048-1).
Code availability: -