| Expression pattern: |
DN |
| Associated gene: |
SIRT1, USP22, Ago2 |
| Associated microRNA: |
miR-3681-3p, miR-5195-3p |
| Biological function: |
inhibits cardiac hypertrophy and promotes autophagy |
| Molecular mechanism: |
Post-transcriptionally upregulates SIRT1 by sponging miR-3681-3p/miR-5195-3p and post-translationally stabilizes SIRT1 by recruiting USP22 to promote deubiquitination of SIRT1 |
| Biological pathway or process: |
autophagy (promotes); apoptosis (not specified); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; IF (Immunofluorescence); In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
In cardiac hypertrophy models, circ-SIRT1 is down-regulated and acts as an anti-hypertrophic factor. It promotes autophagy and suppresses hypertrophy by increasing SIRT1 via miR-3681-3p/miR-5195-3p sponging and by recruiting USP22 to deubiquitinate and stabilize SIRT1 protein. |
| Confidence score: |
0.7726 |