| Expression pattern: |
UP |
| Associated gene: |
KLF4 |
| Associated microRNA: |
miR-7-5p |
| Biological function: |
promotes proliferation; promotes stemness |
| Molecular mechanism: |
Acts as a miR-7-5p sponge, thereby relieving repression of KLF4. |
| Biological pathway or process: |
proliferation (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
CDR1as is up-regulated in hepatoblastoma tissues/cell lines and in CSC-enriched spheres. Functionally, CDR1as promotes HB cell proliferation and cancer stemness by acting as a ceRNA that sponges miR-7-5p, thereby increasing KLF4 expression; CDR1as knockdown suppresses tumor growth in a HepG2 xenograft model. |
| Confidence score: |
0.8634 |