| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
miR-192-3p |
| Biological function: |
Inhibits CRC cell proliferation and migration; promotes apoptosis; affects cell cycle (G0/G1 arrest upon overexpression). |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-192-3p (supported by bioinformatics prediction and dual-luciferase assay). |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); apoptosis (promotes); cell cycle (other); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Sanger Sequencing; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Luciferase Reporter Assay; ROC Analysis |
| Clinical significance: |
Potential diagnostic biomarker (AUC 0.71, sensitivity 0.72, specificity 0.70). |
| Description: |
hsa_circ_0043278 is downregulated in colorectal cancer tissues and cell lines and functions as a tumor suppressor by inhibiting proliferation and migration while promoting apoptosis and G0/G1 arrest. Mechanistically, it sponges miR-192-3p (validated by dual-luciferase assay) and shows moderate diagnostic potential (AUC 0.71). |
| Confidence score: |
0.7855 |