| Expression pattern: |
DN |
| Associated gene: |
SRSF1, DHX9, VEGFA |
| Associated microRNA: |
- |
| Biological function: |
Tumor-suppressive effects in GBM, including decreasing GBM cell migration and reducing pro-angiogenic potential; its reduced binding to SRSF1 increases migration and angiogenesis-related outputs (VEGFA splicing ratio and secreted VEGFA mRNA). |
| Molecular mechanism: |
circSMARCA5 acts as an RBP decoy binding SRSF1 via GAUGAA motif-containing sites, thereby regulating SRSF1-mediated splicing outcomes (VEGFA isoforms) and downstream migration/angiogenesis phenotypes; DHX9 is proposed as a negative regulator of circSMARCA5 expression. |
| Biological pathway or process: |
migration (inhibits); angiogenesis (inhibits); other pathway/process (other) |
| Detected method: |
Q
B
|
| Validation methods: |
Northern Blot; RT-qPCR; Clinical Sample Validation; Cohort Study; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Tube Formation Assay; Survival Analysis |
| Clinical significance: |
circSMARCA5 expression positively correlates with overall patient survival and progression free survival in GBM. |
| Description: |
circSMARCA5 is down-regulated in GBM and functions as a tumor-suppressive RBP decoy for SRSF1. Disrupting its GAUGAA motif weakens SRSF1 binding and increases GBM cell migration and pro-angiogenic activity, associated with altered VEGFA isoform balance and increased extracellular VEGFA mRNA. |
| Confidence score: |
0.7513 |