| Expression pattern: |
UP |
| Associated gene: |
TCF12, PI3K, AKT, Ago2 |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
Promotes glioma cell proliferation, invasion, EMT and tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
Acts as a ceRNA to sponge miR-515-5p, thereby upregulating TCF12 and activating the PI3K/AKT pathway. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; CCK8; BrdU; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circPIP5K1A expression is associated with shorter survival and malignant clinicopathological features; it is a potential prognostic marker in glioma. |
| Description: |
CircPIP5K1A is up-regulated in glioma and promotes malignant behaviors (proliferation, invasion, EMT, tumor growth) while inhibiting apoptosis. Mechanistically, it sponges miR-515-5p to upregulate TCF12 and activate the PI3K/AKT pathway, and higher circPIP5K1A is associated with worse patient survival. |
| Confidence score: |
0.8169 |