| Expression pattern: |
UP |
| Associated gene: |
SOX5, Ago2 |
| Associated microRNA: |
miR-219a-5p |
| Biological function: |
Promotes osteosarcoma cell proliferation, colony formation, migration, invasion, EMT, and tumor growth in vivo; knockdown suppresses these phenotypes. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-219a-5p, thereby relieving miR-219a-5p-mediated suppression of SOX5. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
Higher circ_0007534 level is associated with advanced stage (III-IV) and lymph node metastasis; proposed potential diagnostic/therapeutic target. |
| Description: |
hsa_circ_0007534 (circ_0007534) is up-regulated in osteosarcoma tissues and cell lines and functions as an oncogenic circRNA. It promotes OS proliferation, migration/invasion, EMT and tumor growth by sponging miR-219a-5p, leading to increased SOX5 expression. |
| Confidence score: |
0.7548 |