circRNA basic information
circBase ID: hsa_circ_0000479
Name: hsa_circ_EPSTI1
Synonym: circEPSTI1
Host Gene: EPSTI1
Genomic location(hg19): chr13:43528083-43544806:-
Genomic location(hg38): chr13:42953947-42970670:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
4362
Disease MeSH ID:
-
Disease NCIt ID:
C9311
Disease ICD11 ID:
1256072522
Disease OMIM ID:
603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues

Cell lines:

CaSki; HeLa; HcerEpic

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: SLC7A11, GPX4, Ago2
Associated microRNA: miR-375, miR-409-3P, miR-515-5p
Biological function: Promotes cervical cancer cell proliferation and tumor growth; inhibits ferroptosis.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-375, miR-409-3P and miR-515-5p to regulate SLC7A11 and ferroptosis-related redox balance (GSH/GSSG, lipid peroxidation, GPX4).
Biological pathway or process:

proliferation (promotes); ferroptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; BrdU; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

circEPSTI1 might act as a new and useful biomarker for monitoring and treatment target for cervical cancer.

Description:

circEPSTI1 is up-regulated in cervical cancer cell lines and promotes proliferation and xenograft tumor growth. Mechanistically, it acts as a ceRNA that sponges miR-375/miR-409-3P/miR-515-5p to increase SLC7A11, thereby inhibiting ferroptosis (reduced lipid peroxidation and maintained GSH/GPX4). It is proposed as a potential biomarker and therapeutic target in cervical cancer.

Confidence score:

0.7081

Other information
Title:

Circular RNA circEPSTI1 accelerates cervical cancer progression via miR-375/409-3P/515-5p-SLC7A11 axis.

Journal: Aging
Published: 2021
PubMed ID: 33534779
Study type:

biological research

Data availability: -
Code availability: -