| Expression pattern: |
UP |
| Associated gene: |
SOX4 promoter, NURF complex, SNF2L, BPTF, RBBP4 |
| Associated microRNA: |
- |
| Biological function: |
Promotes gastric cancer cell proliferation, migration and invasion; inhibits apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Nuclear circ-DONSON associates with the NURF chromatin-remodeling complex (via direct interaction with SNF2L) and recruits it to the SOX4 promoter to increase chromatin accessibility/active histone marks and activate SOX4 transcription. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); other pathway/process (promotes) |
| Detected method: |
Q
B
H
M
|
| Validation methods: |
Microarray; RT-qPCR; Northern Blot; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; Colony Formation Assay; EdU Staining; IF (Immunofluorescence); Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; ChIP / ChIP-seq; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
Elevated circ-DONSON is positively associated with advanced TNM stage, lymph node metastasis, and unfavorable prognosis; high expression correlates with lower overall survival and disease-free survival. |
| Description: |
circ-DONSON (hsa_circ_0004339) is upregulated in gastric cancer and its high expression is associated with advanced stage and poor survival. Functionally, it promotes proliferation, migration/invasion and tumor growth while inhibiting apoptosis. Mechanistically, nuclear circ-DONSON binds SNF2L and recruits the NURF complex to the SOX4 promoter to activate SOX4 transcription. |
| Confidence score: |
0.8419 |