| Expression pattern: |
UP |
| Associated gene: |
LPAATbeta / lysophosphatidic acid acyltransferase beta |
| Associated microRNA: |
miR-340-5p |
| Biological function: |
Promotes cisplatin resistance in osteosarcoma cells; circ-CHI3L1.2 knockdown decreases cisplatin IC50 and multidrug-resistance protein levels, promotes apoptosis, induces MET, and suppresses migration and invasion. |
| Molecular mechanism: |
circ-CHI3L1.2 acts through a ceRNA mechanism by targeting/sponging miR-340-5p and regulating the miR-340-5p-LPAATbeta axis. |
| Biological pathway or process: |
chemoresistance (promotes); drug resistance (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; Bioinformatics Analysis |
| Clinical significance: |
Targeted delivery of circ-CHI3L1.2 siRNA to cisplatin-resistant osteosarcoma cells may be a potentially useful clinical treatment strategy; no clinical sample validation was performed. |
| Description: |
circ-CHI3L1.2 is up-regulated in cisplatin-resistant osteosarcoma cell lines compared with parental cells. Functionally, circ-CHI3L1.2 promotes cisplatin resistance, migration, invasion and EMT-like features while suppressing apoptosis; its knockdown sensitizes resistant cells to cisplatin. Mechanistically, it acts as a ceRNA for miR-340-5p to regulate the LPAATbeta axis. |
| Confidence score: |
0.717 |