| Expression pattern: |
DN |
| Associated gene: |
ASXL1 |
| Associated microRNA: |
miR-3187-3p |
| Biological function: |
suppresses proliferation; enhances apoptosis; induces G1 arrest |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-3187-3p to upregulate ASXL1 (via ASXL1 5′-UTR), thereby inhibiting CRC cell proliferation. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; RNA-seq; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
circ-ITGA7 might be a potential diagnostic biomarker and a therapeutic target for CRC. |
| Description: |
circ-ITGA7 is significantly down-regulated in colorectal cancer tissues and CRC cell lines. It suppresses CRC cell proliferation by acting as a ceRNA sponge for miR-3187-3p, relieving repression of ASXL1 (via ASXL1 5′-UTR), and it promotes apoptosis and G1-phase arrest in vitro. |
| Confidence score: |
0.7933 |