| Expression pattern: |
UP |
| Associated gene: |
ANTXR 1 / ANTXR1 |
| Associated microRNA: |
miR-6894-5p |
| Biological function: |
circAFF2 promotes gastric cancer cell proliferation, migration, invasion, and tumor growth in vivo; knockdown of circAFF2 suppresses these malignant phenotypes. |
| Molecular mechanism: |
circAFF2 acts as a sponge for miR-6894-5p and regulates/activates ANTXR 1 expression, forming the circAFF2/miR-6894-5p/ANTXR1 axis in gastric cancer progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Luciferase Reporter Assay; Bioinformatics Analysis; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
Upregulated circAFF2 expression was relevant to depth of tumor invasion, TNM stage, and tumor size, and circAFF2 may act as a potential new biomarker and therapeutic target of gastric cancer. |
| Description: |
circAFF2 is up-regulated in gastric cancer tissues, plasma, and cell lines. It promotes GC cell proliferation, migration, invasion, and xenograft tumor growth by sponging miR-6894-5p and relieving repression of ANTXR1. Its expression is associated with tumor invasion depth, TNM stage, and tumor size, suggesting biomarker and therapeutic-target potential. |
| Confidence score: |
0.8314 |