| Expression pattern: |
DN |
| Associated gene: |
PIK3CA, Sirt1, AKT, p-AKT, Bad, Bax, Caspase-9, cleaved-Parp, cleaved-caspase-3, Bcl-2 |
| Associated microRNA: |
miR-320a, miR-136 |
| Biological function: |
circHECTD1 inhibits LPS-induced apoptosis of alveolar epithelial cells and alleviates LPS-induced acute lung injury in mice. |
| Molecular mechanism: |
circHECTD1 acts as a miRNA sponge for miR-320a and miR-136, thereby regulating PIK3CA and Sirt1 expression and downstream apoptosis-related signaling in LPS-induced ALI. |
| Biological pathway or process: |
apoptosis (inhibits); PI3K/AKT (promotes); ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circHECTD1 may be a therapeutic target of ALI. |
| Description: |
circHECTD1 is down-regulated in LPS-induced human and mouse alveolar epithelial cells and in LPS-induced ALI mouse lung tissues. Its overexpression suppresses alveolar epithelial cell apoptosis and alleviates mouse ALI by sponging miR-320a and miR-136, thereby up-regulating PIK3CA and Sirt1-related anti-apoptotic pathways. |
| Confidence score: |
0.6585 |