circRNA basic information
circBase ID: hsa_circ_0000199
Name: hsa_circ_AKT3
Synonym: circRNA AKT3
Host Gene: AKT3
Genomic location(hg19): chr1:243708811-243736350:-
Genomic location(hg38): chr1:243545509-243573048:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast cancer
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; adjacent normal tissues; TNBC tissues; non-TNBC tissues
Cell lines:

MCF-10A; MDA-MB-231; MDA-MB-468; SK-BR-3; MCF-7
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: PI3K/Akt/mTOR signaling
Associated microRNA: miR-613, miR-206
Biological function: facilitates chemo-tolerance/chemo-resistance and promotes proliferation, migration and invasion; modulates autophagy; reduces chemosensitivity in TNBC cells
Molecular mechanism: ceRNA sponging of miR-613 and miR-206 leading to activation of PI3K/Akt/mTOR signaling
Biological pathway or process:

PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); autophagy (other); ceRNA regulation (promotes); chemoresistance (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Survival Analysis; Luciferase Reporter Assay; Transfection; MTT; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

high hsa_circ_0000199 expression was predictive of large tumor size, advanced TNM grade, high Ki-67 level and poor 3-year survival of TNBC patients
Description:

hsa_circ_0000199 (AKT3-derived) is up-regulated in TNBC and promotes malignant phenotypes and chemoresistance. It sponges miR-206 and miR-613 to modulate PI3K/AKT/mTOR signaling, affecting proliferation, migration/invasion, chemosensitivity and autophagy, and its high expression predicts poor survival in TNBC patients.
Confidence score:

0.7126
Other information
Title:

Hsa_circ_0000199 facilitates chemo-tolerance of triple-negative breast cancer by interfering with miR-206/613-led PI3K/Akt/mTOR signaling.

Journal: Aging
Published: 2021
PubMed ID: 33495420
Study type:

combined biological and clinical study
Data availability: All data generated or analyzed during this study are included in this article.
Code availability: -