circRNA basic information
circBase ID: -
Name: hsa_circ_NOLC1
Synonym: Circ-NOLC1
Host Gene: NOLC1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005140
MONDO name: ovarian carcinoma
Disease details: epithelial ovarian cancer
Disease DO ID:
 4001
Disease MeSH ID:
 -
Disease NCIt ID:
 C4908
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

normal ovaries; benign ovarian tumors; borderline tumors; ovarian cancer tissues / ovarian carcinoma tissues
Cell lines:

A2780; CAOV3; ES-2; HO8910; OVCAR3; SKOV3; HEK-293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ESRP1, RhoA, CDK1
Associated microRNA: miR-326-5p, miR-330, miR-370, miR-9-5p
Biological function: promotes cell proliferation, migration and invasion; inhibits apoptosis; promotes tumor growth in vivo
Molecular mechanism: binds ESRP1 (RBP interaction) and increases ESRP1, CDK1 and RhoA expression; miRNA-sponge hypothesis tested but luciferase suggests no direct binding to candidate miRNAs
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); other pathway/process (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model
Clinical significance:

higher circ-NOLC1 expression was positively associated with FIGO stage, differentiation and CA125 status; may have potential as diagnostic biomarker
Description:

circ-NOLC1 is up-regulated in epithelial ovarian cancer and is associated with more advanced FIGO stage, poorer differentiation, and CA125 positivity. Functionally, it promotes proliferation, migration and invasion and inhibits apoptosis in vitro, and enhances tumor growth in a cell line-derived xenograft model. Mechanistically, it binds the RBP ESRP1 (validated by RIP) and increases ESRP1, CDK1 and RhoA expression, supporting an ESRP1-mediated regulation of CDK1/RhoA rather than a direct miRNA-sponge effect for the tested miRNAs.
Confidence score:

0.6833
Other information
Title:

Circ-NOLC1 promotes epithelial ovarian cancer tumorigenesis and progression by binding ESRP1 and modulating CDK1 and RhoA expression.

Journal: Cell death discovery
Published: 2021
PubMed ID: 33483472
Study type:

combined biological and clinical study
Data availability: Supplementary material (available to authorized users) / Supplemental file
Code availability: -