circRNA basic information
circBase ID: hsa_circ_0000936
Name: hsa_circ_SHKBP1
Synonym: -
Host Gene: SHKBP1
Genomic location(hg19): chr19:41089303-41089623:+
Genomic location(hg38): chr19:40583397-40583717:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma microvessels; normal brain microvessels
Cell lines:

hCMEC/D3; U87MG; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FOXP1, FOXP2, AGGF1, Ago2
Associated microRNA: miR-544a, miR-379
Biological function: promotes endothelial cell viability, migration, and tube formation (angiogenesis) in glioma-exposed endothelial cells
Molecular mechanism: Acts as a miRNA sponge in an Ago2/RISC manner to sequester miR-544a and miR-379, thereby increasing FOXP1/FOXP2 and downstream AGGF1; AGGF1 activates PI3K/AKT and ERK1/2 signaling to promote angiogenesis.
Biological pathway or process:

angiogenesis (promotes); proliferation (promotes); migration (promotes); PI3K/AKT (promotes); ERK (promotes); ceRNA regulation (promotes)
Detected method:
Q
H
Validation methods:

RT-qPCR; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Tube Formation Assay; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

-
Description:

circ-SHKBP1 is up-regulated in glioma microvessels and glioma-exposed endothelial cells and promotes angiogenic phenotypes (viability, migration, tube formation). Mechanistically, it sponges miR-544a and miR-379 (Ago2/RISC-dependent), releasing FOXP1/FOXP2 to transcriptionally upregulate AGGF1, which activates PI3K/AKT and ERK1/2 signaling to drive angiogenesis.
Confidence score:

0.7977
Other information
Title:

circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways.

Journal: Molecular therapy. Nucleic acids
Published: 2018
PubMed ID: 29499945
Study type:

combined biological and clinical study
Data availability: Supplemental Information includes two figures and can be found with this article online at https://doi.org/10.1016/j.omtn.2017.12.014.
Code availability: -