| Expression pattern: |
UP |
| Associated gene: |
DDHD2, AGO2 |
| Associated microRNA: |
miR-296-3p |
| Biological function: |
promotes proliferation; promotes migration; promotes invasion; promotes tumorigenesis; promotes metastasis |
| Molecular mechanism: |
ceRNA mechanism: circRUNX1 sponges miR-296-3p to upregulate DDHD2 |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; divergent primers PCR; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; RNA-seq; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
circRUNX1 expression level was significantly higher in patients with larger tumor size, advanced TNM stage, extrathyroidal extension and lymph node metastasis; might therefore be considered a novel prognostic biomarker |
| Description: |
circRUNX1 is up-regulated in papillary thyroid cancer and promotes proliferation, migration and invasion in vitro and tumor growth in vivo. Mechanistically, circRUNX1 mainly localizes in the cytoplasm and acts as a ceRNA by sponging miR-296-3p, thereby relieving repression of DDHD2 and driving PTC progression. |
| Confidence score: |
0.8265 |