circRNA basic information
circBase ID: hsa_circ_0003221
Name: hsa_circ_PTK2
Synonym: CircPTK2
Host Gene: PTK2
Genomic location(hg19): chr8:141856358-141900868:-
Genomic location(hg38): chr8:140846259-140890769:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005595
MONDO name: laryngeal squamous cell carcinoma
Disease details: laryngeal squamous cell carcinoma
Disease DO ID:
 2876
Disease MeSH ID:
 -
Disease NCIt ID:
 C4044
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

LSCC tissues; adjacent normal tissues
Cell lines:

SNU899; SNU46; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: YAP1, Ago2
Associated microRNA: miR-1278
Biological function: promotes LSCC cell viability/proliferation and cell cycle progression; inhibits apoptosis; promotes tumor growth in vivo
Molecular mechanism: Acts as a ceRNA (molecular sponge) for miR-1278 to upregulate YAP1 expression
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Agarose Gel Electrophoresis; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Clinical Sample Validation; Cohort Study; In Vivo Animal Model
Clinical significance:

Higher hsa_circ_0003221 level was closely related to clinical stage and lymph-node metastasis in LSCC patients
Description:

circPTK2 (hsa_circ_0003221), derived from PTK2, is up-regulated in LSCC tissues and cell lines and is associated with advanced clinical stage and lymph-node metastasis. Functionally, circPTK2 promotes proliferation/cell-cycle progression and suppresses apoptosis, and its knockdown inhibits xenograft tumor growth. Mechanistically, circPTK2 acts as a ceRNA sponging miR-1278 to upregulate YAP1 in LSCC cells.
Confidence score:

0.8405
Other information
Title:

CircPTK2 (hsa_circ_0003221) Contributes to Laryngeal Squamous Cell Carcinoma by the miR-1278/YAP1 Axis.

Journal: Journal of oncology
Published: 2021
PubMed ID: 34691176
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -