| Expression pattern: |
UP |
| Associated gene: |
MTDH |
| Associated microRNA: |
miR-1298-5p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion and tube formation (angiogenesis) and inhibits apoptosis; promotes xenograft tumor growth. |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-1298-5p to upregulate MTDH expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circPKM2 is upregulated in NSCLC tissues and cell lines and mainly localizes to the cytoplasm. It promotes NSCLC malignant phenotypes and xenograft growth by sponging miR-1298-5p, thereby increasing MTDH expression. |
| Confidence score: |
0.8443 |