| Expression pattern: |
UP |
| Associated gene: |
CFL2 |
| Associated microRNA: |
miR-1299 |
| Biological function: |
promotes viability, metastasis, and proliferation; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA sponge mechanism: circ_0006404 binds miR-1299 and relieves repression of CFL2 |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; MTT; Transwell Assay; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
Circ_0006404 might be a stable potential bio-marker for PCa diagnosis and treatment. |
| Description: |
circ_0006404 (circFOXO3) is up-regulated in prostate cancer tissues and cell lines, promotes malignant phenotypes (proliferation, migration/invasion) and inhibits apoptosis. Mechanistically, it sponges miR-1299 to increase CFL2 expression, promoting tumor growth in a DU145 xenograft model. |
| Confidence score: |
0.7979 |