circRNA basic information
circBase ID: hsa_circ_0076305
Name: hsa_circ_PGC
Synonym: circPGC
Host Gene: PGC
Genomic location(hg19): chr6:41704448-41712252:-
Genomic location(hg38): chr6:41736710-41744514:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissue; paracancerous tissue
Cell lines:

AGS
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: MMP9, MMP14, Collagen I, E-cadherin, Vimentin, beta-catenin, Snail, Twist, EGFR, PCNA, c-Myc
Associated microRNA: -
Biological function: inhibits proliferation; promotes migration and invasion; promotes epithelial-to-mesenchymal transition; promotes extracellular matrix/basement membrane degradation
Molecular mechanism: Overexpression of circPGC decreases EGFR, PCNA and c-Myc and decreases E-cadherin while increasing Vimentin, beta-catenin, Snail and Twist; upregulates MMP9, MMP14 and Collagen I, consistent with migration-proliferation dichotomy and EMT-related changes.
Biological pathway or process:

proliferation (inhibits); migration (promotes); invasion (promotes); EMT (promotes)
Detected method:
Q
Validation methods:

Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot
Clinical significance:

circPGC expression is lower in clinical patients with later stages of GC, which is associated with poor prognosis.
Description:

In gastric cancer, circPGC (hsa_circ_0076305) is down-regulated in tumors and decreases further in advanced stages. In AGS cells, circPGC overexpression suppresses proliferation but promotes migration/invasion and EMT-like changes, accompanied by increased MMP9/MMP14/Collagen I and reduced EGFR/PCNA/c-Myc/E-cadherin.
Confidence score:

0.5751
Other information
Title:

Hsa_circ_0076305 induces migration-proliferation dichotomy in gastric cancer.

Journal: Oncology letters
Published: 2021
PubMed ID: 33613709
Study type:

combined biological and clinical study
Data availability: GSE100170
Code availability: -