| Expression pattern: |
UP |
| Associated gene: |
MYD88, LEF1, IRAK-4, NF-кB signaling pathway |
| Associated microRNA: |
miR-665 |
| Biological function: |
promotes ECM degradation, inhibits chondrocyte proliferation, and induces apoptosis in osteoarthritis progression |
| Molecular mechanism: |
circRNF121 sponges miR-665 to indirectly upregulate MYD88 and activate NF-кB signaling; LEF1 promotes circRNF121 expression by binding RNF121 promoter |
| Biological pathway or process: |
NF-kappaB (promotes); apoptosis (promotes); proliferation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RIP (RNA Immunoprecipitation); FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
The expression of circRNF121 was positively associated with Mankin’s scores. |
| Description: |
In osteoarthritis cartilage/chondrocytes, circRNF121 is up-regulated and promotes OA progression by increasing ECM degradation, inducing apoptosis, and suppressing proliferation. Mechanistically, circRNF121 acts as a ceRNA that sponges miR-665 to upregulate MYD88 and activate NF-kappaB signaling; its expression is promoted by the transcription factor LEF1. |
| Confidence score: |
0.7799 |