| Expression pattern: |
UP |
| Associated gene: |
NAP1L3 |
| Associated microRNA: |
miR-498 |
| Biological function: |
promotes proliferation and invasion; promotes tumor growth and progression |
| Molecular mechanism: |
circGFRA1 acts as a miRNA sponge for miR-498 to up-regulate NAP1L3 |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; BrdU; Colony Formation Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
High circGFRA1 expression was markedly related to tumor size, intrahepatic metastasis, extrahepatic metastasis, BCLC stage and TNM stage as well as poor overall survival. |
| Description: |
circGFRA1 is up-regulated in HCC tissues and cell lines and is associated with advanced stage and poor overall survival. Functionally, circGFRA1 promotes HCC proliferation, invasion, and xenograft tumor growth by sponging miR-498 to increase NAP1L3 expression. |
| Confidence score: |
0.7891 |