circRNA basic information
circBase ID: hsa_circ_0032683
Name: hsa_circ_NEK9
Synonym: CircNEK9
Host Gene: NEK9
Genomic location(hg19): chr14:75590748-75590926:-
Genomic location(hg38): chr14:75124045-75124223:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; paracancerous normal tissues; plasma; plasma exosomes; xenograft tumor tissues
Cell lines:

XGC-1; MKN45; GES1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MAP7, MMP2, MMP9
Associated microRNA: miR-409-3p
Biological function: CircNEK9 promotes gastric cancer cell proliferation, cell cycle progression, migration, invasion, metastasis-related protein expression, xenograft tumor growth, and exosome-mediated motility of recipient GC cells.
Molecular mechanism: CircNEK9 functions as a ceRNA by sponging miR-409-3p, thereby relieving miR-409-3p-mediated repression of MAP7 and promoting GC progression; plasma exosomal circNEK9 also enhances malignant motility of recipient GC cells.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); exosome (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model
Clinical significance:

High circNEK9 expression was associated with large tumor size and advanced TNM stage; plasma exosomal circNEK9 might be a novel marker for GC diagnosis and treatment.
Description:

CircNEK9 is up-regulated in gastric cancer tissues, cell lines, and plasma exosomes. It promotes GC proliferation, cell cycle progression, migration, invasion, metastasis, and xenograft tumor growth by sponging miR-409-3p and up-regulating MAP7. Plasma exosomal circNEK9 may serve as a diagnostic or therapeutic marker and enhances malignant motility of recipient GC cells.
Confidence score:

0.78
Other information
Title:

Plasma Exosomal CircNEK9 Accelerates the Progression of Gastric Cancer via miR-409-3p/MAP7 Axis.

Journal: Digestive diseases and sciences
Published: 2021
PubMed ID: 33449227
Study type:

combined biological and clinical study
Data availability: GSE78092; GSE93541; TCGA COAD
Code availability: -