| Expression pattern: |
UP |
| Associated gene: |
HDAC4 |
| Associated microRNA: |
miR-637 |
| Biological function: |
promotes OS cell proliferation, migration and invasion |
| Molecular mechanism: |
circHIPK3 acts as a miRNA sponge for miR-637, thereby promoting HDAC4 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; Luciferase Reporter Assay; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circHIPK3 had clinical value in OS (area under the curve, 0.8750; P<0.0001) and high circHIPK3 expression was closely associated with shorter overall survival of patients with OS |
| Description: |
circHIPK3 is up-regulated in osteosarcoma tissues and cell lines and mainly localizes to the cytoplasm. It promotes osteosarcoma cell proliferation, migration and invasion by sponging miR-637 and thereby increasing HDAC4 expression; high circHIPK3 expression is associated with shorter overall survival and shows diagnostic value by ROC analysis. |
| Confidence score: |
0.8343 |