| Expression pattern: |
UP |
| Associated gene: |
HIF-1alpha |
| Associated microRNA: |
miR-28-5p |
| Biological function: |
promotes proliferation, migration, and invasion; inhibits apoptosis; promotes xenograft tumor growth; accelerates glycolysis |
| Molecular mechanism: |
ceRNA mechanism: circAGFG1 sponges miR-28-5p to increase HIF-1alpha level and regulate glycolytic metabolism |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Survival Analysis; ROC Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; In Vivo Animal Model |
| Clinical significance: |
High expression of circAGFG1 was closely related to the low survival rate of NSCLC patients; diagnostic value (AUC 0.918) in tumor tissues vs adjacent normal tissues |
| Description: |
circAGFG1 is up-regulated in NSCLC tissues and serum and is associated with poor overall survival. Functionally, circAGFG1 promotes NSCLC cell proliferation, migration, invasion, glycolysis and tumor growth while inhibiting apoptosis, mainly by sponging miR-28-5p to increase HIF-1alpha. |
| Confidence score: |
0.8198 |