| Expression pattern: |
UP |
| Associated gene: |
ABCC2, Ago2 |
| Associated microRNA: |
miR-665 |
| Biological function: |
promotes HCC cell proliferation and invasion, and inhibits apoptosis |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge to decoy miR-665 and thereby regulate ABCC2 expression |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circABCC2 could be used as a biomarker and therapeutic target in HCC. |
| Description: |
circABCC2 is up-regulated in HCC tissues and cell lines and promotes malignant phenotypes (proliferation and invasion) while inhibiting apoptosis. Mechanistically, circABCC2 is mainly cytoplasmic and functions as a ceRNA by sponging miR-665 to increase ABCC2 expression, thereby promoting HCC progression in vitro and in xenograft models. |
| Confidence score: |
0.742 |